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Registro Completo |
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
17/05/2022 |
Data da última atualização: |
17/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FREIRE FILHO, F. R.; ROCHA, M. de M.; BRIOSO, P. S. T.; RIBEIRO, V. Q. |
Afiliação: |
FRANCISCO RODRIGUES FREIRE FILHO, CPAMN; MAURISRAEL DE MOURA ROCHA, CPAMN; PAULO SÉRGIO TORRES BRIOSO, UFRJ; VALDENIR QUEIROZ RIBEIRO, CPAMN. |
Título: |
'BRS Guariba': white-grain cowpea cultivar for the mid-north region of Brazil. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 6, p. 175-178, 2006. |
Idioma: |
Inglês |
Conteúdo: |
BRS Guariba is a cultivar that belongs to the white commercial class, with a early-maturing cycle and resistance to powdery mildew (Erysiphe polygoni), brown blotch (Coletrichum truncatum) and Cowpea aphid borne mosaic virus-CABMV. It attained mean grain yields of 1475 and 1508 kg ha-1 in the states of Piauí and Maranhão, respectively. It is indicated for cultivation in the Mid-North region of Brazil. |
Palavras-Chave: |
Feijão Caupi. |
Thesagro: |
Semente; Variedade; Vigna Unguiculata. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143097/1/BRSGuaribaWhiteGrainCowpeaCBAB6.2006.pdf
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Marc: |
LEADER 01011naa a2200205 a 4500 001 2143097 005 2022-05-17 008 2006 bl uuuu u00u1 u #d 100 1 $aFREIRE FILHO, F. R. 245 $a'BRS Guariba'$bwhite-grain cowpea cultivar for the mid-north region of Brazil.$h[electronic resource] 260 $c2006 520 $aBRS Guariba is a cultivar that belongs to the white commercial class, with a early-maturing cycle and resistance to powdery mildew (Erysiphe polygoni), brown blotch (Coletrichum truncatum) and Cowpea aphid borne mosaic virus-CABMV. It attained mean grain yields of 1475 and 1508 kg ha-1 in the states of Piauí and Maranhão, respectively. It is indicated for cultivation in the Mid-North region of Brazil. 650 $aSemente 650 $aVariedade 650 $aVigna Unguiculata 653 $aFeijão Caupi 700 1 $aROCHA, M. de M. 700 1 $aBRIOSO, P. S. T. 700 1 $aRIBEIRO, V. Q. 773 $tCrop Breeding and Applied Biotechnology$gv. 6, p. 175-178, 2006.
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Embrapa Meio-Norte (CPAMN) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
10/05/2022 |
Data da última atualização: |
10/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; ALMEIDA, N. N. C.; FIGUEIRO, M. R.; QUINTAO, C. C. R.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; NATHALIA N. C. ALMEIDA, Universidade Federal do Pará; MARIVALDO RODRIGUES FIGUEIRO, CPATU; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAQUIM M. GARCIA, Universidade Estadual Paulista. |
Título: |
Epigenetic modifiers during in vitro maturation as a strategy to increase oocyte competence in bovine. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Theriogenology, v. 187, p. 95-101, 2022. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2022.04.014 |
Idioma: |
Inglês |
Conteúdo: |
Oocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-implantation development when used at low concentrations. MenosOocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-... Mostrar Tudo |
Palavras-Chave: |
Cromatina; Epigenética; Oocito; Tricostatina A. |
Thesagro: |
Bovino; Embrião; Reprodução Animal. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02377naa a2200277 a 4500 001 2142833 005 2022-05-10 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2022.04.014$2DOI 100 1 $aSARAIVA, N. Z. 245 $aEpigenetic modifiers during in vitro maturation as a strategy to increase oocyte competence in bovine.$h[electronic resource] 260 $c2022 520 $aOocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-implantation development when used at low concentrations. 650 $aBovino 650 $aEmbrião 650 $aReprodução Animal 653 $aCromatina 653 $aEpigenética 653 $aOocito 653 $aTricostatina A 700 1 $aOLIVEIRA, C. S. 700 1 $aALMEIDA, N. N. C. 700 1 $aFIGUEIRO, M. R. 700 1 $aQUINTAO, C. C. R. 700 1 $aGARCIA, J. M. 773 $tTheriogenology$gv. 187, p. 95-101, 2022.
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